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Reduced coronary vasodilatation and increased vasoconstriction to diadenosine pentaphosphate after ischemia‐reperfusion in isolated rat heart
Author(s) -
GarciaVillalon Angel Luis,
Fernandez Nuria,
Monge Luis,
Salcedo Adely,
Narvaez Raul,
Dieguez Godofredo
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.730.33
Subject(s) - vasoconstriction , vasodilation , medicine , perfusion , ischemia , cardiology , coronary perfusion pressure , anesthesia , cardiopulmonary resuscitation , resuscitation
To analyze the role of diadenosine polyphosphates in coronary ischemia‐reperfusion, the coronary response to diadenosine pentaphosphate (AP5A, 10 −7 – 10 −5 M) was recorded in isolated perfused rat hearts after precontraction of the coronary vasculature with U46619 (10 −8 –3×10 −8 ). Under control, AP5A injected intracoronarily produced a small initial increase in perfusion pressure (vasoconstriction, 4±2; 7±2; 14±5 mmHg of increment) followed by a decrease in perfusion pressure (vasodilatation, 21±7; 66±1; 52±18 mmHg of decrease), together with a reduction in both left ventricle contractility (dP/dt, 162±37; 764±182; 1388±392 mmHg/s of reduction) and heart rate. After 30 min of ischemia followed by 15 min of reperfusion, the vasoconstriction produced by AP5A was greater (13±2; 20±3; 47±6 mmHg of increment in perfusion pressure, P<0.01), while the vasodilatation was lower (0±3; 15±5; 1±8 mmHg of reduction in perfusion pressure, P<0.05), than in control. The antagonist of purinergic P 2Y receptors reactive blue (2×10 −6 M) inhibited the vasodilatation and potentiated the vasoconstriction both in control and after ischemia‐reperfusion. Therefore ischemia‐reperfusion may impair the coronary vasodilatation to AP5A mediated by purinergic P 2Y receptors and may enhance vasoconstriction. This later effect might be a consequence of the decreased vasodilatation (Supported, in part, by FMMA, FIS and MEyC)