Premium
α‐CGRP diminishes infarct size in a murine model of myocardial ischemia‐reperfusion injury
Author(s) -
Katki Khurshed A,
Bhardwaj Ashwani K,
DiPette Donald J,
Supowit Scott C
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.730.17
Subject(s) - calcitonin gene related peptide , medicine , endocrinology , reperfusion injury , ischemia , myocardial infarction , calcitonin , troponin complex , troponin i , anesthesia , neuropeptide , receptor
The efferent function of the peripheral sensory nervous system is mediated by the release of neuropeptides, such as α‐calcitonin gene‐related (CGRP). CGRP, a potent vasodilator, has been suggested to play a beneficial role in myocardial ischemia/reperfusion (I/R) injury. Therefore, we examined the role of α‐CGRP in I/R injury, using a mouse model of acute myocardial I/R. CGRP knockout (KO; n=6/group) and wild type (WT; n=6/group) mice received 30 minutes of occlusion of the left anterior descending artery, followed by 24 hours of reperfusion. Hearts were evaluated for infarct size by Evan's Blue and TTC staining. Plasma troponin and cardiac isoprostanes were also assessed. After I/R, the area at risk (AAR) was increased 55% in both the KO and WT mice. The α‐CGRP KO mice exhibited a significantly increased area of infarct (IA; 35%) compared to their WT counterparts (28%). Plasma troponin, a marker of ischemic injury, was elevated in both KO (0.8±0.4 ng/ml) and WT (0.9±0.6 ng/ml) mice after I/R, compared to the respective sham animals (KO, 0.5±0.08 ng/ml; WT, 0.5±0.2 ng/ml). The α‐CGRP KO mice exhibited a 30% rise in cardiac isoprostanes (a marker of oxidative stress) compared to sham animals, while the WT mice showed an increase of 20% compared to sham animals. Therefore, these data indicate that deletion of α‐CGRP gene makes the heart vulnerable to I/R injury possibly due, at least in part, to increased oxidative stress.