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Activation of p38 MAP kinase is required for acrylamide‐inhibited NCAM expression in SK‐N‐SH neuroblastoma cells.
Author(s) -
Lee Hyerim,
Son Eunwha,
Rhee DongKwon,
Pyo Suhkneung
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.717.4
Subject(s) - neural cell adhesion molecule , acrylamide , western blot , p38 mitogen activated protein kinases , electrophoretic mobility shift assay , mapk/erk pathway , blot , microbiology and biotechnology , neuroblastoma , kinase , gene isoform , chemistry , transcription factor , biology , cell , cell adhesion , biochemistry , cell culture , gene , genetics , organic chemistry , copolymer , polymer
Acrylamide is a well‐known industrial neurotoxic chemical that can induce neurodegeneration. Neural cell adhesion molecule (NCAM) is cell surface macromolecules that belong to the immunoglobulin(Ig) superfamily, which is expressed as three main isoforms‐NCAM‐120, NCAM‐140 and NCAM‐180. We investigated the effect of acrylamide on NCAM expression in human neuroblastoma cell (SK‐N‐SH), assessed by RT‐PCR, EMSA and western blot analysis. The results show that treatment of cells with acrylamide resulted in the decrease of protein and mRNA levels of NCAM in a time‐ and dose‐dependent manner. We next examined the possible roles of the MAP kinase family on acrylamide‐treated cells, because these kinases family have been described as playing a critical role in cellular signal pathways induced by a variety of environmental stresses. Western blot analysis showed that the activation of p38 was induced in response to acrylamide in a dose‐dependent manner, whereas it did not affect JNK and ERK activity. In addition, electrophoretic mobility shift assay indicated that acrylamide dose‐dependently decreased the DNA binding affinity of the Ikaros transcription factor. Taken together, these results indicate that activation of p38 but not of ERK and JNK is essential for acrylamide‐inhibited NCAM expression of SK‐N‐SH cells and acrylamide influences transcriptional stage of NCAM expression via ikros activation.