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Contributions of extracellular and intracellular calcium in the biosynthesis of endocannabinoids
Author(s) -
Placzek Ekaterina A,
Barker Eric L
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.711.3
Subject(s) - anandamide , endocannabinoid system , extracellular , 2 arachidonoylglycerol , biosynthesis , intracellular , calcium , chemistry , biochemistry , calcium in biology , microbiology and biotechnology , cannabinoid , cannabinoid receptor , biology , enzyme , receptor , antagonist , organic chemistry
The actions of a plant‐derived cannabinoid delta 9 ‐tetrahydrocannabinol (δ 9 ‐THC) are mimicked by the endogenous cannabinoids anandamide (AEA) and 2‐arachidonoylglycerol (2‐AG). The mechanisms that underlie AEA and 2‐AG biosynthesis are not completely understood. It is known that endocannabinoid biosynthesis is a calcium‐dependent process, however the physiological source of calcium is not known. We performed a series of biochemical experiments examining the contributions of extracellular and intracellular calcium in the biosynthetic process. We discovered that extracellular calcium is necessary for AEA and 2‐AG biosynthesis, whereas simply releasing intracellular calcium stores does not have an effect on endocannabinoid biosynthesis. The model for endocannabinoid biosynthesis was extended into MES 23.5 cells, a neuronal cell line. We determined that AEA biosynthesis in MES 23.5 cells may occur as a result of the activation of TRPC 3/6/7‐type channels.