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Maturational alterations of proliferation predominance of Hirsutella sinensis and Paecilomyces hepiali in natural Cordyceps sinensis
Author(s) -
Zhu JiaShi,
Guo Yinglan,
Lu Jihong
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.704.5
Subject(s) - cordyceps , camellia sinensis , horticulture , degree (music) , chemistry , botany , biology , zoology , physics , acoustics
Cordyceps sinensis (Cs) has been used for centuries in China as a medicinal herb for Yin‐Yang double‐invigorations. We reported co‐existence of Paecilomyces hepiali (Ph) and Hirsutella sinensis (Hs) in Cs (FESAB 19:A1033, 2005; 21:A1079, 2007). In this study, 206 pieces of fresh Cs were collected in Kangding, Sichuan (Qinghai‐Tibetan Plateau) in mid May (0–10 degree C) and late June (10–20 degree C). Of them, 120 pieces were immature Cs (T1) with a plump caterpillar body (CB) and a short stroma (Stm); and 86 were mature Cs (T2) with a less plump CB and a long Stm. Fresh Cs were washed and surface sterilized in 0.1% Mercuric Chloride for 2–10 min, followed by washing (3×) with sterile water. CB and Stm of 206 pieces of Cs were individually cut into pieces (1.5∼2.0 mm thick), and placed on top of slant PDAP culture medium. Culture was done at 10 degree C. Growth of Hs colonies was found in 82 tubes (39.8%) of Cs tissue slides: 61 (50.8%) of T1 Cs and 21 (24.4%) of T2 Cs (p<0.001). At the unfavorable low culture temperature, Ph was grown in 12 tubes (5.83%) of Cs tissue slides: 5.83% for T1 Cs and 5.81% for T2 (p>0.50). In conclusion, the proliferation predominance of Hs decreases along with Cs maturation, and of Ph needs to be further checked at an optimal temperature for Ph.