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Intrinsic carbon‐13 labeling of red cabbage anthocyanins for human nutrition studies
Author(s) -
Charron Craig S.,
Britz Steven J.,
Mirecki Roman M.,
Harrison Dawn J.,
Clevidence Beverly A.,
Novotny Janet A.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.701.7
Subject(s) - chemistry , anthocyanin , red cabbage , cyanidin , food science , absorption (acoustics) , ingestion , horticulture , metabolism , botany , biochemistry , biology , materials science , composite material
Although health benefits of dietary anthocyanins (ACN) have received significant attention, scientific progress has been slowed by an incomplete understanding of ACN absorption and metabolism. Thus, we developed a system to produce carbon‐13 labeled ACNs in red cabbage to enable study of ACN fate after ingestion. Twenty‐eight ‘Super Red’ red cabbage seedlings were grown in an inorganic growth medium in 10.1 cm pots. Upon appearance of the first true leaves, pots were placed in a gas‐tight acrylic box located in a walk‐in growth chamber. Nutrient solution was delivered hydroponically. Temperature was maintained at 22.0 ± 0.5 °C until day 26, then at 10.5 ± 1.5 °C until plants were harvested on day 34. 13 CO 2 concentration in the box was monitored with an infrared gas analyzer, and 13 CO 2 (99.3% pure) was injected when concentration was < 390 μL L −1 . ACNs were analyzed by HPLC‐MS/MS. Carbon sites of the predominant nonacylated ACN, cyanidin 3‐diglucoside‐5‐glucoside, and the predominant acylated ACN, cyanidin 3‐(feruloyl)(sinapoyl) diglucoside‐5‐glucoside, were > 98% labeled. This high incorporation rate of 13 C favors precise tracing of ACNs and ACN metabolites in upcoming feeding studies. Funded by the USDA/ARS.