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Dehydrocostus lactone (DHCL) isolated from the root of Saussurea lappa inhibits the migration and invasion of DU145 human prostate cancer cells
Author(s) -
Hong Ji Eun,
Kim Eun Ji,
Lim Soon Sung,
Shin HyunKyung,
Kim JongSang,
Park Jung Han Yoon
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.700.40
Subject(s) - du145 , chemistry , cell migration , secretion , viability assay , metastasis , matrix metalloproteinase , in vitro , adhesion , cancer cell , pharmacology , biochemistry , cancer , biology , medicine , lncap , organic chemistry
Saussurea lappa has been used in traditional herbal medicine and shown to exert anti‐inflammatory and anti‐carcinogenic effects. In present study, we investigated the effects of the hexane extracts of SL (HE SL ) on the migration and invasion characteristics of DU145 cells. DU145 cells were cultured in the presence of 0 – 4 μg/mL HE SL with or without 10 ng/mL EGF. HE SL inhibited the basal and EGF‐induced cell migration, invasion and adhesion in dose‐dependent manners. Active fractions were isolated by column chromatography and the structure of the active principle was determined using 1 H NMR and 13 C NMR. The active compound DHCL inhibited the basal and EGF‐induced migration, invasion and cell adhesion in dose‐dependent manners. DHCL decreased secretion of matrix metalloproteinase (MMP)‐9 and tissue inhibitor of metalloproteinase (TIMP)‐1 but increased TIMP‐2 levels in concentration‐dependent manners. We have shown that HE SL and its active principle DHCL inhibit the metastasis potential of DU145 human prostate cancer cells. Our results indicate that inhibition of MMP‐9 secretion and stimulation of TIMP‐2 expression contribute to decreased migration and invasion of DU145 cells treated with HE SL and DHCL.