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Apoptosis induced by piplartine on PC‐3 cells
Author(s) -
KONG Eun Hee,
An Soon Cheol,
Meydani Mohsen
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.700.38
Subject(s) - apoptosis , annexin , dapi , prostate cancer , mtt assay , cancer research , chemistry , pharmacology , biology , cancer , microbiology and biotechnology , medicine , biochemistry
Prostate cancer treatment is androgen ablation of frequently option but this treatment has limited scope, especially, for hormone‐refractory cancer. Piplartine, a main component of Piper species have known anti‐proliferation and anti‐cancer activity. We investigated the effects of piplartine on PC‐3 cells by using the MTT assay, DAPI staining, PI staining assay, Annexin V binding assay, FACS analysis and Western bloting. The apoptosis in the PC‐3 cells is induced by piplartine and the caspase‐3‐mediated PARP cleavage is involved in the apoptosis process. In addition, the treated cells arrest at a G2 point in the cell cycle might be involved in apoptosis induced by piplartine. In conclusion, these results provide the molecular basis for this potent leading inhibitors to act as anti‐tumor agents in chemotherapy for human prostate cancer and piplartine may be a lead candidate compound to explore anticancer drug potential.