DNA Repair in Human Lymphocytes by Sinigrin ‐ a Possible Mechanism of Action: Comet –FLARE Assay.

Chronic low level exposure to environmental pollutants such as synthetic pesticides has been implicated in many health conditions such as oxidative stress, leading to DNA damage, a cause behind many serious diseases such as cancer. The objective of the present study was to investigate the possible mode of action of Sinigrin (10–30μM) a bio‐active compound found in vegetables such as broccoli, cabbage, Brussels sprouts, in the DNA repair process, using Single Cell Gel Electrophoresis (Comet Assay) ‐ Fragment Length Analysis using Repair Enzymes (FLARE) assay. Human lymphocytes were exposed to various concentrations of Sinigrin (10–30 μM) along with (a) a mixture of most widely used pesticides and (b) 100 mM of H2O2 incubated simultaneously at 37° C, at 5% CO2 for 3 hours and DNA repair was evaluated using formamidopyrimidine FLARE‐Comet Assay. The efficiency of Sinigrin in protecting DNA damage was found at and above 20 μM, suggesting a possible role in the functioning of DNA glycosylase involved in DNA repair process.