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Dietary proteins alter tissue‐specific gene expression and adiposity in male Sprague‐Dawley rats
Author(s) -
Simmen Frank A,
Greenway Amy D,
Bowman Margaret T,
Su Ying,
Kang Ping
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.700.2
Subject(s) - endocrinology , medicine , adipose tissue , soy protein , biology , gene expression , casein , jejunum , gene , biochemistry
Dietary soy protein elicits anti‐obesity effects in rodents. This study examined adiposity and gene expression in male Sprague‐Dawley rats lifetime‐fed diets containing casein (CAS), soy protein isolate (SPI), or casein supplemented with genistein (250 mg/kg diet; GEN). At 48 days of age, retroperitoneal fat weight (normalized to BW) was reduced in SPI vs. CAS and GEN groups. Expression profiling identified 28 SPI up‐regulated and 52 SPI down‐regulated jejunum transcripts (relative to CAS). A subset of these genes was examined for dietary protein regulation in jejunum, liver and abdominal fat pad. In jejunum, Stearoyl‐CoA desaturase 2 (Scd2) and Kruppel‐like factor 15 (Klf15) mRNAs were SPI‐induced and Malic enzyme 1 (Me1) and Iodothyronine deiodinase type I (Dio1) mRNAs were SPI‐suppressed (compared to CAS), with no effects of GEN observed. In liver, Scd2, thyroid receptors (TR)alpha 1 and beta 1, and Dio1 were SPI‐induced and Me1 was SPI‐suppressed, with no effects of GEN observed. In adipose tissue, Klf15 and Klf9 mRNAs were suppressed by GEN but not SPI. Results identify metabolic (Me1, Scd2), transcriptional (Klf15, Klf9, TRs), and thyroid hormone system (TRs, Dio1) genes affected in complex tissue‐specific fashion by SPI that may contribute to anti‐obesity in rats. These newly described effects of SPI appear to be unrelated to the major soy isoflavone GEN. Supported by CRIS 6251‐51000‐005‐3S