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Effects of vegetable‐derived phytochemicals on differentiation‐associated factors in 3T3‐L1 preadipocytes
Author(s) -
CHANG HSIAOPEI,
CHEN YUEHWA
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.700.16
Subject(s) - adiponectin , cruciferous vegetables , adipocyte , chemistry , 3t3 l1 , phenethyl isothiocyanate , triglyceride , allyl isothiocyanate , oil red o , endocrinology , medicine , isothiocyanate , biochemistry , adipose tissue , biology , adipogenesis , cholesterol , cancer , insulin resistance , insulin
The study was aimed at investigating the effect of various vegetable‐derived phytochemicals, including indole‐3‐carbinol (I3C), phenethyl isothiocyanate (PEITC) and benzyl isothiocyanate (BEITC) from cruciferous vegetable, diallyl sulfide (DAS) from garlic, as well as capsaicin from red pepper, on the various factors related to adipocyte differentiation in 3T3‐L1 preadipocytes. The results indicated that these phytochemicals not only dose‐dependently suppressed tumor necrosis factor‐£\ (TNF‐£\)‐interferon£^‐lipopolysaccharide‐stimulated NO production in the undifferentiated 3T3‐L1 preadipocytes, but also inhibited intracellular lipid accumulation and triglyceride contents in the differentiated 3T3‐L1 adipocytes. Additionally, the secretion of IL‐6 and adiponectin by differentiated adipocytes was also modulated by the treatment of these phytochemicals. In conclusion, phytochemicals from cruciferous vegetable, garlic and red pepper are able to dysregulate preadipocyte differentiation by inhibiting lipid and triglyceride accumulation, and such suppression is associated with various adipocyte differentiation mediators, including NO, IL‐6, and adiponectin,