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Experimental in vitro arterial reactivity and tissue culture solutions alter the time dependent stability of anthocyanins from chokeberry, bilberry and elderberry extracts.
Author(s) -
Bell David Robert,
Nalliah Ruth Ellen,
Phillips Jordan S,
Gaier Abby J,
Jones Francis L,
Gochenaur Kristen E
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.699.1
Subject(s) - anthocyanin , chemistry , in vitro , monomer , bilberry , polymerization , biochemistry , food science , organic chemistry , polymer
Acute exposure to anthocyanin enriched extracts from chokeberry (Ch), bilberry (B) and elderberry (E) produces endothelium‐dependent, NO mediated vasorelaxation of porcine coronary arteries. Extracts at 0.05mgTa/L also attenuate impaired vasorelaxation resulting from external or internal oxidant stress. Determining the stability of these extracts in in vitro settings is necessary to evaluate their long term effects on arteries. Therefore, we examined the stability of monomeric anthocyanins from Ch, B and E in common in vitro arterial function solutions. Reference extract solutions (0.75mg/ml in DD H2O at 21C)showed less than 25% monomeric anthocyanin degradation over a period of 9 days as determined by the pH differential method. Solutions made with a physiological salt solution (PSS) used for in vitro arterial reactivity studies showed significantly greater degradation at 21C.(1/2 life, hrs: Ch= 13, E=35, B=4.6). Degradation in PSS was increased 3–4 fold at 37C and 2 fold more with 95%O2/5%CO2 added. Similar results were seen in Dulbecco's modified Eagle's media solutions at 37C. Analysis of degradation by the bisulfite method revealed time dependent anthocyanin polymerization in in vitro arterial solutions. Thus solutions used to study arterial function in vitro produce significant degradation and polymerization of monomeric anthocyanins from Ch, B and E. These factors must be taken into account in the design and interpretation of studies that examine long term effects of anthocyanins on arterial function in vitro. (Supported by IUSM REGrant #39407‐1090 and the Lutheran Foundation of Indiana Grant # 6721712)