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Differential analysis of the serum proteome from bull terrier pups with lethal acrodermatitis
Author(s) -
Grider Arthur,
Casal Margret L
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.697.13
Subject(s) - proteome , silver stain , blood proteins , albumin , acrodermatitis enteropathica , biology , staining , pathology , proteomics , biomarker , medicine , endocrinology , physiology , zinc deficiency (plant disorder) , bioinformatics , biochemistry , gene , micronutrient
The biochemical lesion in bull terriers that causes lethal acrodermatitis (LAD) has not been identified, though the phenotype is similar to the systemic zinc deficiency syndrome in humans, acrodermatitis enteropathica. Currently, there is no biomarker for the disease. The goal of this research is to determine whether differential analysis of control and affected dog serum proteomes will identify proteins that might be used as biomarkers for the disease. Serum was obtained from two affected pups (15.6 wks of age) and one control (17.1 wks of age). Cibachron blue spin columns were used to remove albumin. The samples (100 g protein) were prepared for two‐dimensional gel electrophoresis. Following staining with colloidal Coomassie Blue, the gel images were analyzed for differential protein expression. Five proteins were increased in the serum from the unaffected bull terrier pup and three proteins exhibited increased expression in the serum from the affected bull terrier pups. These proteins may serve as biomarkers to identify LAD heterozygotes. The identifications of the differentially expressed proteins will be determined using peptide mass fingerprinting following mass spectrometry and database analysis. Funded in part by NCRR 2P40RR002512‐19 and USDA Hatch GEO00513.