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Expression of selenoprotein K in different tissues and cell types
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.696.11
Subject(s) - messenger rna , spleen , biology , endoplasmic reticulum , kidney , selenoprotein , microbiology and biotechnology , endocrinology , medicine , immunology , gene , biochemistry , oxidative stress , glutathione peroxidase , catalase
Selenoprotein K (Sel K) is a resident endoplasmic reticulum protein of unknown function. As a first step of characterizing this protein, we assessed the relative expression of Sel K mRNA by real‐time PCR in different murine tissues including brain, heart, intestine, kidney, liver, lung, spleen, and testes. Results showed that the abundance of Sel K mRNA was relatively high in all tissues. Because Sel K was particularly abundant in spleen, we examined mRNA abundance in purified murine and human CD4 + T cells and found that levels were also relatively high and did not change upon activation of the cells. Sel K is also highly expressed in heart and we examined mRNA levels in heart tissue from hyperthyroid vs. normal mice and found no significant difference between the two conditions. Our results suggest that Sel K is a ubiquitously expressed selenoprotein and levels remain stable in response to T cell activation or myocardial hypertrophy. Studies are underway to determine whether Sel K protein levels in different tissues and under different conditions reflect those found for mRNA.