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Ventral midbrain vs. striatal iron infusion to correct altered in vivo dopaminergic signaling in ID rats
Author(s) -
Bianco Laura Elizabeth,
Rundle Sarah E,
Ghramm Lindsay H,
Beard John L
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.694.4
Subject(s) - dopaminergic , striatum , microdialysis , chemistry , extracellular , endocrinology , in vivo , medicine , transferrin receptor , transferrin , dopamine , biology , biochemistry , microbiology and biotechnology
Iron deficiency (ID) results in altered monoamine functioning in vivo including decreased DA transporter (DAT) and D 2 receptor densities, decreased intracellular DA, increased extracellular DA and altered communication between D 2 and DAT. We attempted to remediate DA functioning in the striatum by rescuing iron status in the cell bodies (Ventral Midbrain‐VMB) or the terminal fields (Striatum‐ST) by iron infusion. Using in vivo microdialysis and no net flux we found that infusion of 26 pmols iron into the ST resulted in no change in striatal extraction fraction or D 2 R – DAT communication in either CN or ID. However, infusion of iron into the VMB resulted in decreased E d as well as remediation of D 2 R‐DAT in ID rats. Although local increases in iron could not be detected by AA, transferrin receptor levels were decreased in the ST after iron infusion indicating iron was delivered to the target areas. Additionally, ELISA measurements found that striatal intracellular DA, extracellular DA, and TH levels were also returned to normal levels after VMB but not ST infusion in ID rats. These data indicate that appropriate iron status in the VMB is required for proper striatal dopaminergic functioning.