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Soluble cellulose reduces expression of lipogenic and inflammatory genes in fat fed hamsters
Author(s) -
Hong YunJeong,
Bartley Glenn,
Turowski Maciej,
Anderson Kerr,
Yokoyama Wallace
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.691.9
Subject(s) - adipose tissue , medicine , endocrinology , adiponectin , glut4 , cd36 , inflammation , chemistry , adipocyte , leptin , hamster , insulin resistance , biology , insulin , receptor , obesity
The underlying cause of the metabolic differences between normal and insulin resistant hamsters fed identical high fat diets except for dietary fiber solubility were investigated by determining relative expression of genes for fat metabolism and inflammation. Male Syrian hamsters switched from standard laboratory chow to a high fat diet become insulin resistant and hyperlipidemic. If the same high fat diet contains hydroxypropylmethylcellulose, as the dietary fiber source, most of the adverse physiological effects of the high fat diet are ameliorated. Relative expression of genes related to fat metabolism, nuclear receptors, and inflammation were determined in order to understand the molecular basis for the normalization of blood and liver lipids and adipose mass. Primers for hamster leptin, adiponectin, CYP51, FXRalpha, CD36, F4/80, MAC‐1, GLUT4, SCD1, TNF alpha, PPAR alpha and gamma, SREBP‐1c and others in liver and/or adipose were developed. Dietary soluble cellulose consistently lowered SCD1 and increased CYP51 expression relative to insoluble microcrystalline fiber feeding. Adipose GLUT4 and inflammatory marker expression was decreased. PPARalpha expression but not PPARgamma increased in some studies.