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25‐hydroxyvitamin D inhibits differentiation of 3T3‐L1 preadipocytes
Author(s) -
Natarajan Radhika,
Ronnenberg Alayne G,
Wood Richard J,
Lee OkHwan,
Kim YoungCheul
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.691.2
Subject(s) - adipogenesis , adipocyte , endocrinology , vitamin d and neurology , medicine , prohormone , 3t3 l1 , adipose tissue , cellular differentiation , cell culture , hormone , chemistry , lipid droplet , biology , microbiology and biotechnology , biochemistry , gene , genetics
Adipogenesis is a key process in the development of obesity as well as normal development and function of adipose tissue. Some evidence in humans suggests an inverse association between adiposity and serum 25‐hydroxyvitamin D (25‐D), the circulating form of vitamin D. Recent studies in the murine 3T3‐L1 preadipocyte cell line indicate that 1,25‐dihydroxyvitamin D (1,25‐D), the active hormonal form of vitamin D, inhibits adipogenesis, but no studies have investigated the role of 25‐D, the prohormone form, which is converted to 1,25‐D by CYP27B1. PURPOSE: This study was designed to investigate whether 25‐D treatment affects adipocyte differentiation in murine 3T3‐L1 cells. METHODS: 3T3‐L1 preadipocytes were treated with various concentrations of 1,25‐D and 25‐D for up to 6 days following standard induction of differentiation. The degree of differentiation, lipid accumulation (oil red O) and expression of various adipogenic genes was assessed. RESULTS: Both 1,25‐D and the 25‐D prohormone inhibited adipocyte differentiation, lipid accumulation and expression of the key adipogenic transcription factor PPARγ. CONCLUSION: 25‐D is biologically active in 3T3‐L1 cells, which is consistent with a possible direct role of vitamin D status on fat cell biology. The effects of 25‐D may reflect the intracellular conversion of the prohormone to the active 1,25‐D hormone form in differentiating preadipocytes.