Premium
A medicinal plant extract of Scutellaria baicalensis and Acacia catechu reduced LPS stimulated gene expression in immune cells: A comprehensive genomic study using QPCR, ELISA, and microarray
Author(s) -
TsengCrank Julie,
Sung Sookyung,
Jia Qi,
Zhao Yuan,
Burnett Bruce,
Yimam Mesfin,
Park DaeRyoung,
Woo SungSick
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.691.13
Subject(s) - scutellaria baicalensis , microarray , microarray analysis techniques , biology , chemokine , microbiology and biotechnology , tumor necrosis factor alpha , gene expression , immune system , gene , immunology , biochemistry , medicine , alternative medicine , pathology , traditional chinese medicine
A standardized flavonoid extracts from Scutellaria baicalensis and Acacia catechu, UP446 (Univestin™), was profiled favorably in its anti‐inflammatory properties. In this study, quantitative polymerase chain reaction (QPCR), enzyme‐linked immunosorbent assay (ELISA), and cDNA microarray were used to study the effect of UP446 on lipopolysaccharide (LPS)‐induced pro‐inflammatory reactions. One consistent result was that the gene expression levels stimulated or suppressed by LPS were normalized by UP446 co‐treatment. This normalization effect was observed for the well‐known pro‐inflammatory genes cyclooxygenase (COX)‐2, tissue necrosis factor (TNF)‐ 1 □, interleukin (IL)‐1β, NFκB, and IL‐6 using QPCR and TNF‐α using ELISA. This was also observed by microarray. In addition to the above genes, microarray results also included cytokines, chemokines, chemokine receptor, immunoglobulin receptors, transcriptional factors and coactivator, caspase, growth factors, and phosphatases. The microarray results supported not only the anti‐inflammatory property of UP446, but also suggested that UP446 may participate in additional signaling pathways of cell proliferation, cell death and lipid metabolism. This research work was supported by Unigen.