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Metabolomics analysis of plasma from humans depleted of choline
Author(s) -
Sha Wei,
Berger Alvin,
Costa KerryAnn da,
Zeisel Steven H.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.688.8
Subject(s) - choline , metabolomics , metabolism , medicine , analysis of variance , chemistry , endocrinology , physiology , biology , chromatography
In previous studies, we measured targeted metabolites in choline and 1‐carbon metabolism in plasma from humans depleted of choline. In this study, we utilized an untargeted metabolomic approach to globally determine the biochemicals affected by a choline deficient diet. Adult men and women with different genetic profiles in genes involved in folate and choline metabolism were fed a diet containing 550 mg choline/d for 10 d. They were then fed a choline deficient diet for up to 42 d. Fifteen subjects who were deemed clinically choline deficient (developed liver and muscle dysfunction) on the low choline diet were then repleted with graded amounts of choline. Blood was collected at the end of each phase, and plasma analyzed using LC‐MS. ANOVA was used to compare metabolomic profiles in the subjects to select biochemicals that significantly changed their abundance (p<0.1) in the following pair‐wise comparisons: baseline vs. depleted; depleted vs. repleted; and baseline vs. repleted. We found 38 named biochemicals significantly changed by choline depletion and 26 changed by choline repletion in various biochemical classes. We are currently examining biochemicals that change in subjects grouped by organ dysfunction, gender and genetic polymorphism, and applying multivariate statistical approaches on both named and un‐named biochemicals. This research is supported by grants from NIH‐DK55865, DK56350 and RR00046.