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Eicosapentaenoic acid ameliorates lipid accumulation via up‐regulation of lipolytic gene expression and down‐regulation of adipogenic gene expression in 3T3‐L1 adipocytes
Author(s) -
Lee MakSoon,
Kim ChongTai,
Cho YongJin,
Kim ChulJin,
Kim Yangha
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.687.4
Subject(s) - adipogenesis , 3t3 l1 , lipolysis , cd36 , lipid droplet , eicosapentaenoic acid , peroxisome , chemistry , carnitine , lipid metabolism , fatty acid , fatty acid synthase , gene expression , medicine , endocrinology , biochemistry , biology , adipose tissue , receptor , polyunsaturated fatty acid , gene
In this study, the lipolytic effect of eicosapentaenoic acid (EPA) was investigated in 3T3‐L1 adipocytes. In comparison with the control, intracellular lipid accumulation was significantly decreased by 24% after 24 hours following the addition of EPA. A 61% increase of glycerol release into the medium occurred with the addition of EPA in the differentiated 3T3‐L1 adipocytes. Also, free fatty acids release into the medium increased by 44.6% compared to the control. The mRNA level of carnitine palmitoyltransferase I‐a (CPTI‐a), a component of the fatty‐acid shuttle system involved in the mitochondrial oxidation of long‐chain fatty acids, was significantly increased by EPA. However, the expression of peroxisome proliferator‐activated receptor‐γ (PPAR‐γ) and acetyl‐CoA carboxylase (ACC), which are involved in adipogenesis, was significantly down‐regulated by EPA. These results suggest that EPA may ameliorate lipid accumulation by stimulation of lipolysis accompanied with induction of lipolytic gene expression and suppression of adipogenic gene expression in 3T3‐L1 adipocytes. “This work supported by the Food Nanotechnology Development Project (2007) and KOSEF (No. M10510130005‐07N1013‐00510).”