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WAT Gene Expression of IL‐1ra Predicts Hepatic Steatosis in Obese Balb/c mice and is Reduced by Behavioral Intervention
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.685.3
Subject(s) - adipokine , steatosis , medicine , endocrinology , leptin , sed , adipose tissue , white adipose tissue , triglyceride , obesity , analysis of variance , inflammation , cholesterol
Obesity increases adipokine secretion from white adipose tissue (WAT), which is correlated with hepatic steatosis (HS). Exercise has been shown to decrease HS but the mechanism is not clear. We sought to determine if exercise training is associated with decreased WAT inflammation, and if this predicts lower HS in obese mice. Mice (n=34) fed a 45% fat diet (HFD) for 12 wks were randomly assigned to an exercise group (EX: TM running, 5d/wk X 12 wks; 60 min/d; 12m/min; 5% grade; 65–70%VO 2 ) or remained sedentary (SED). Mice remained on HFD or were placed on a 10% fat diet (LFD) for a 12 wk intervention. Following the intervention, tissue samples were frozen in liquid N 2 and stored at −80°C. HS was assessed by measuring total liver triglyceride (TG) content. Real time PCR was used to determine WAT gene expression of adipokines (IL‐1ra, leptin, MCP‐1, F4/80, IL‐6). Using ANOVA, LFD reduced IL‐1ra (25%) and F4/80 (49.6%), while EX reduced MCP‐1 (52.5%) (p<0.05; no significant interactions). Both interventions improved HS. Using linear regression, IL‐1ra significantly predicted HS (β=0.35, p=0.042) independent of all of the other cytokines and body weight % gain from baseline. Body weight also predicted HS (β=0.50, p<0.001) (Reg. R 2 =0.72; F=7.88, p<0.001). IL‐1ra is a new adipokine that has been shown to have deleterious systemic effects. These data suggest that IL‐1ra may also exacerbate HS in diet‐induced obesity.