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Effect of intermittent fasting on markers of oxidative stress and serum biochemical markers of cellular damage in healthy subjects
Author(s) -
Ibrahim Wissam H.,
Habib Hosam M.,
Jarrar Amjad H.,
Albaz Samer A.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.678.20
Subject(s) - malondialdehyde , oxidative stress , medicine , endocrinology , uric acid , albumin , glutathione peroxidase , glutathione , creatine kinase , vitamin e , catalase , carotenoid , vitamin c , antioxidant , chemistry , biochemistry , enzyme
During the lunar month of Ramadan Muslims abstain from eating, drinking, and smoking from dawn to sunset. This makes Ramadan a unique model for studying the effects of intermittent fasting in humans. The aim of this study was to determine the effect of Ramadan fasting on markers of oxidative stress and serum biochemical markers of cellular damage in healthy subjects. Fourteen healthy volunteers (9 men and 5 women aged 25 – 58) who fasted during Ramadan participated in the study. Blood sampling was conducted 2 days before Ramadan, the 14th and 28th days of Ramadan. The following were measured: 1) Serum: malondialdehyde, AST, ALT, creatine kinase, ALP, LDH, BUN, total proteins, uric acid, albumin, glucose, triglycerides, and total cholesterol, 2) Plasma: protein‐bound carbonyls, alpha‐tocopherol, gamma‐tocopherol, retinol, vitamin C, and carotenoids, 3) Erythrocytes: MDA, glutathione, glutathione peroxidase and catalase. Erythrocyte malondialdehyde, serum glucose and triglycerides, and plasma total carotenoids were significantly lower (P < 0.05) at the 28th day of Ramadan compared to before Ramadan. The rest of the variables were not significantly altered by Ramadan fasting. The results obtained indicate that Ramadan fasting confers protection against lipid peroxidative damage in erythrocytes as noted by significant decreases in malondialdehyde and does not adversely affect cellular damage.