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Evaluating gene expression patterns induced by SEB and LPS in human PBMCs
Author(s) -
Mendis Chanaka,
Jett Marti
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.675.3
Subject(s) - gene , enterotoxin , biology , dna microarray , microarray , microarray analysis techniques , gene expression , exotoxin , toxin , gene expression profiling , real time polymerase chain reaction , computational biology , genetics , escherichia coli
Both Staphylococcal enterotoxin B (SEB), an exotoxin produced by staphylococcal aureus and lipopolysaccharide (LPS) released from various bacterial cell walls are commonly associated with food poisoning and capable of triggering flu‐like symptoms. Here we compared the host response to these distinct toxins at a genetic level by investigating the time dependent expression pattern of a set of genes that have been known to explain some of the symptoms induced by the toxins through their functions. After obtaining a specific gene expression pattern for each toxin through microarray analysis a set of genes were selected for further analysis based on their function, such as apoptosis, cytokine induction, DNA binding, phosphorylation or transporter activity. After designing primers for the selected genes, real time PCR and RT‐PCR were carried out to confirm the genetic expression pattern initially established by microarray analysis. The combination of these gene analysis techniques such as microarrays, real‐time PCR and RT‐PCR will provide us the opportunity to effectively and accurately identify/confirm a set of toxin‐specific genes as well as to gain some useful information of the mechanism of action.