Premium
Th2 cell differentiation first occurs in the T:B zone of the lymph node and is associated with specific innate immune cell populations
Author(s) -
Rozo Cristina T,
Liu Qian,
Gause William
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.674.10
Subject(s) - biology , lymph node , immune system , immunology , microbiology and biotechnology , in vivo , cellular differentiation , b cell , innate immune system , interleukin 4 , antibody , genetics , gene
The cell populations and molecules involved in triggering the development of Th2 cells remain uncertain. Our previous studies have shown that in vivo Th2 cell differentiation occurs in the absence of IL‐4 production by non‐T cells. Using in situ cytokine detection methodologies and laser microdissection, transferred DO11.10 Th2 cell differentiation was tracked in IL‐4 KO recipient mice and the T:B zone of the draining lymph node was identified as the microenvironment where in vivo Th2 cell differentiation first occurs as early as 3–4 days after intracutaneous N. Brasiliensis inoculation. To examine in vivo what innate immune cell populations contribute to the development of IL‐4 producing T cells in the T:B zone microenvironment, antigen presenting cells were identified with specific antibodies that detect Ag complexed to MHCII and with DQ‐OVA, which fluoresces following Ag processing. We observed that under Th2 conditions, interstitial DCs present Ag and that DX5+ basophils accumulate as early as 12 hours after immunization, but do not present Ag. Further analysis of basophils is currently being conducted to determine their contribution to the development of the Th2 response.