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Human defensin alpha‐1 causes Trypanosoma cruzi membrane pore formation and induces DNA fragmentation, which leads to trypanosome destruction
Author(s) -
Madison Marisa Nia,
Kleshchenko YY,
Nde PN,
Simmons KJ,
Lima MF,
Villalta F
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.674.1
Subject(s) - trypanosoma cruzi , defensin , biology , innate immune system , beta defensin , microbiology and biotechnology , immune system , immunology , peptide , biochemistry , parasite hosting , world wide web , computer science
Human defensins play a fundamental role in the initiation of innate immune responses to some microbial pathogens. Here we show that mammalian cells rapidly up‐regulate the expression of human defensin alpha‐1 as an innate response to counter Trypanosoma cruzi infection. We discovered that defensin alpha‐1 displays a trypanocidal role against T. cruzi , the causative agent of Chagas' disease. The toxicity of human defensin alpha‐1 against T. cruzi is mediated by membrane pore formation and the induction of nuclear and mitochondrial DNA fragmentation, leading to trypanosome destruction. Exposure of trypomastigote and amastigote forms of T. cruzi to defensin alpha‐1 significantly reduced parasite viability in a peptide concentration‐dependent and saturable manner. Electron microscopic analysis of trypomastigotes exposed to defensin alpha‐1 revealed pore formation in the cellular and flagellar membranes, membrane disorganization, and blebbing as well as cytoplasmic vacuolization. Furthermore, human defensin alpha‐1 enters the trypanosome when membrane pores are present and is associated with later intracellular damage. Preincubation of trypomastigotes with defensin alpha‐1 followed by exposure to human epithelial cells significantly reduced T. cruzi infection in these cells. Thus, human defensin alpha‐1 is an innate immune molecule that causes severe toxicity to T. cruzi and plays an important role in reducing cellular infection. This is the first report showing that human defensin alpha‐1 causes membrane pore formation in a human parasite, leading to trypanosome destruction. This work was supported in part by NIH grants 1SC1 GM081168,GM 08037, GM 059994, AI 07281, AI 056667, HL 007737, MD 000104,RR 003032, and DK 20539.