Premium
Pet stimulates macrophage and epithelial cells MAPKp38 activity within a different time frame
Author(s) -
BenitezGuzman Alejandro,
Eslava Carlos,
TorresMarquez M Eugenia
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.672.40
Subject(s) - internalization , macrophage , microbiology and biotechnology , p38 mitogen activated protein kinases , mapk/erk pathway , host response , biology , host (biology) , kinase , immunology , immune system , chemistry , cell , in vitro , genetics
Pet belongs to SPATEs toxin family and it is able to trigger several responses on the host. Such consequences to the host or bacteria itself are under aggressive investigation, as to understand how to support host defense mechanisms. NFκB/MAPK p38 mediated is one of the pathways involved in producing pro‐inflammatory interleukins. The kinase seemingly participates in epithelial survival before challenges. Considering that each cellular type conveys different protection functions for the host, the question arises of whether the pattern response induced by Pet is the same. Here, we established the MAPKp38 activation time course in murine macrophages and in HEp‐2 epithelial cells. A biphasic response curve was obtained for both cell types, although one of the activation peaks was different (30 min/12 hours and 30 min/48 h for macrophages and HEp‐2 cells, respectively). The first activity peak is related to external prompting of MAPKp38, while at least in macrophages, the latter response could be associated either with internalized Pet or a response induced by a product of primed macrophages. Studies of Pet internalization pattern for HEp‐2 cells are being carried out.