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Three different subsets of alternatively activated macrophages (M2Mϕ) detected in mice with severe thermal injury
Author(s) -
Shigematsu Kenji,
Kobayashi Makiko,
Herndon David N,
Suzuki Fujio
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.672.33
Subject(s) - ccl17 , medicine , andrology , burn injury , mannose receptor , receptor , tumor necrosis factor alpha , chemokine , immunology , chemistry , chemokine receptor , macrophage , surgery , biochemistry , in vitro
A predominance of M2Mφ in burn mice has been reported in many papers. M2Mφ play a major role on the increased susceptibility of thermally injured hosts to various infections. Since 3 different subsets of M2Mφ (M2a, M2b, M2c) have been recently described, in this study, the properties of M2Mφ appearing in mice with severe thermal injury were examined. Peritoneal Mφ (1 x 10 6 cells/ml, 92% or more purity) were isolated using magnetic beads coated with anti‐F4/80 mAb from mice 1 to 21 days after burn injury (3 rd degree, 25% TBSA burn). Culture fluids of these Mφ, 24 hrs after cultivation without any stimulation, were assayed for CCL5 and IL‐12 (biomarkers of M1Mφ), CCL17 and IL‐10 (biomarkers of M2aMφ), CCL1 and IL‐10 (biomarkers of M2bMφ) and CXCL13 and IL‐10 (biomarkers of M2cMφ) using ELISA. Also, these Mφ preparations were tested for mannose receptor mRNA expression by RT‐PCR. In the results, Mφ from mice 2 to 5 days postburn produced CCL17. Also, Mφ from mice 1 to 14 days postburn produced CXCL13. Mφ from mice 7 to 21 days postburn produced CCL1. Mφ from mice 7 days postburn did not produce CCL17. Mannose receptor mRNA was expressed by Mφ from mice 2 days postburn, while it was not expressed by Mφ from mice 14 days postburn. These results indicate that severe thermal injury induces 3 subsets of M2Mφ with different time courses. M2aMφ are generated in mice early after burn injury (1 to 5 days postburn), M2cMφ are generated in mice 1 to 14 days postburn, and M2bMφ are generated in mice late after burn injury (1 to 3 weeks postburn). This study was supported by SHC NA #8840.