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TSLP is important in the effector phase of allergic skin inflammation
Author(s) -
He Rui,
Oyoshi Michiko,
Garibyan Lilit,
Kumar Lalit,
Ziegler Steven,
geha Raif
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.671.6
Subject(s) - immunology , allergic inflammation , inflammation , ovalbumin , cytokine , thymic stromal lymphopoietin , medicine , antigen , atopic dermatitis
TSLP is a cytokine expressed by keratinocytes and is important in allergic inflammation. Allergic skin inflammation elicited by epicutaneous (EC) immunization of mice with ovalbumin (OVA), which shares many characteristics of the skin lesions of atopic dermatitis (AD), was found to be severely impaired in TSLPR −/− mice, as evidenced by decreased infiltration of eosinophils and decreased local expression of Th2 cytokines. Secretion of Th2 cytokines by splenocytes from EC sensitized mice in response to OVA was normal. Skin dendritic cells from TSLPR −/− mice were normal in their ability to migrate to draining LN, express activation markers and induce proliferation and Th2 cytokine production by naïve T cells. T cells from TSLPR −/− mice expressed the skin homing receptor E‐selectin ligand normally, and homed to the skin normally, but failed to transfer allergic skin inflammation to WT recipients. TSLP enhanced Th2 cytokine secretion by targeting TSLPR on antigen specific T cells. Intradermal injection of anti‐TSLP blocked the development of allergic skin inflammation following antigen challenge of intraperitoneally immunized WT mice. These findings suggest that TSLP is essential for antigen driven Th2 cytokine secretion by skin infiltrating T cells and could be a therapeutic target in allergic skin inflammation