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Trichosanthin induces allergic airway inflammation relying on early IL‐4 production derived from innate immune cells
Author(s) -
Sun Bing,
Wang Yuan,
Mao Kai Rui
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.670.14
Subject(s) - trichosanthin , immunology , inflammation , immune system , innate lymphoid cell , biology , immunoglobulin e , innate immune system , adjuvant , allergic inflammation , antibody , microbiology and biotechnology
Trichosanthin (TCS) is a simple protein isolated from the plant Trichosanthes kirilowii. TCS has anti‐tumor and ant‐HIV activity. Also, it can induce IgE production in both humans and mice. In the present study, we show that TCS can induce airway inflammation, a typical Th2‐type response£without the help of alum adjuvant. Furthermore, TCS is capable of replacing alum to promote OVA‐specific airway inflammation. Interestingly, IL‐4 production in the circulation is associated with an increase in Gr‐1+ cells in peripheral blood. Using IL‐4 neutralizing antibody and IL‐4 gene knockout mice, our sudies show that IL‐4 production at an early stage of OVA‐priming is essential in vivo to create an environment favoring Th2 cell differentiation. Taken together, the data indicate that IL‐4 production from innate immune cells triggered by TCS at an early stage plays a critical role in the pathogenesis of OVA‐specific airway inflammation. Our data suggest that TCS is not only a strong allergen, but also a Th2‐type adjuvant modulating immune response switching to a Th2‐type pathway.