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CD11c+CD11b+ dendritic cells play an important role in intravenous tolerance and suppress ongoing experimental autoimmune encephalomyelitis
Author(s) -
li hongmei,
zhang Guang xian,
Rostami abdolmohamad
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.669.6
Subject(s) - experimental autoimmune encephalomyelitis , cd11c , foxp3 , immunology , il 2 receptor , adoptive cell transfer , immune tolerance , spleen , myelin oligodendrocyte glycoprotein , integrin alpha m , cd8 , dendritic cell , rar related orphan receptor gamma , t cell , immune system , medicine , biology , biochemistry , gene , phenotype
The central role of T cells in the induction of tolerance against experimental autoimmune encephalomyelitis (EAE) has been well documented. However, the role of dendritic cells (DCs), the most potent antigen presenting cells, in this process is not clear. In the present study we address this important question, with particular focus on the differential roles of DC subsets, CD11c+CD11b+ and CD11c+CD8+ DCs, in the induction of tolerance. We found that the proportion of CD11c+CD11b+ DCs was increased in the central nervous system (CNS) and spleen of mice injected intravenously (i.v.) with autoantigen peptide MOG35‐55, which developed significantly less severe EAE than PBS‐injected control mice. These DCs exhibited immunoregulatory characteristics, such as increased production of IL‐10, inhibition of MOG‐induced T cell proliferative responses, and induction of CD4+CD25+Foxp3+ regulatory T cells. More importantly, these DCs significantly suppressed ongoing EAE upon adoptive transfer. Our observations demonstrate that CD11c+CD11b+ DCs, which are abundant in the CNS during induction of tolerance, are crucial for i.v. tolerance induction in EAE and could be exploited for immunotherapy of autoimmune diseases.