Premium
Characterization of the Specificity of Anti‐Neuronal Antibodies in Sera of Multiple Sclerosis Patients
Author(s) -
Takalo Elisa,
Fox Autumn,
DeVries George H.,
Kennett Roger H.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.668.3
Subject(s) - epitope , phage display , antibody , peptide library , biopanning , polyclonal antibodies , microbiology and biotechnology , cdna library , epitope mapping , biology , chemistry , virology , peptide sequence , immunology , gene , biochemistry
Screening of 30 sera from multiple sclerosis (MS) patients against enriched axolemma fractions (AEF) indicated that 11 could be classified as having high titers, 14 as intermediate and 5 as low or negative. (J. of Neurochem. 96 (Suppl. 1), 134) Two of these sera were pooled, diluted, bound to microtiter plates and used to screen a phage display library expressing random peptide epitopes 5–7 amino acids in length. Peptides bound corresponded to sequences in several proteins including ankyrin (3 epitopes), spectrin, K + and Ca ++ ion channels and the extra‐cellular matrix protein AGC1 (2 epitopes). We have continued these experiments by binding antibodies in MS sera to human neuroblastoma cells and using the eluted antibodies to screen a phage display library expressing cDNA clones from human brain. This new procedure detects antibody epitopes on cell surface proteins that may represent targets for antibodies found in MS sera. Screening of the phage display targets with additional MS sera should provide an indication of whether or not they may be involved in the pathogenesis of MS. We thank D. L. Feinstein and C. Kaiser, Department of Anesthesiology, Univ. of IL for the MS sera samples used in the study. The work was supported by Wheaton College Ruth Kraft Strohschein research funds and a VA Merit Grant † .