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Anti‐RNA Antibodies with Differential Specificity towards Polyribonucleotides from a Murine Model of Lupus
Author(s) -
Kole Hemanta,
Bolland Silvia
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.667.1
Subject(s) - antibody , microbiology and biotechnology , rna , antigen , autoantibody , epitope , nucleic acid , chemistry , biology , virology , biochemistry , gene , immunology
Autoantibodies against nucleic acids were found in a lupus prone FcγRIIB −/‐ mice sera in B6 background. With the addition of the yaa (Y‐chromosome autoimmune accelerator) gene, pathogenecity in these male mice was significantly enhanced and autoantibodies switch from anti‐DNA to anti‐RNA specificity. Here we have characterized anti‐RNA antibodies from a hybridoma clone H526 that was produced by the fusion of R2yaa spleenocytes with myeloma cells. Hybridoma H526 produced anti‐RNA IgG2a‐λ antibodies. Sequence analysis showed only a few amino acid changes in the V H and V L regions compared to B6 germ‐line Ig variable regions. H526 antibody was found to deposit in the mouse kidney after 24 hrs of injection. When R2yaa mouse was immunized with H526 antibody, we found a marked reduction of anti‐nucleolar antibodies in the serum indicating neutralization of pathogenic antibodies by anti‐H526 antibody. For the antigenic epitope characterization for the anti‐RNA H526 antibody, we examined many polyribonucleotides of various lengths and found that synthetic polyribonucleotides, Poly (G, C) of 40 mers is optimal for the antibody binding in an ELISA. The Poly (G, C) 40 also selectively binds anti‐RNA antibodies from R2yaa serum. Further characterization of Poly (G, C) in pathogenic B cell binding, detection, and cellular localization and antigen presentation are in progress.