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CD27 plus CD28 Cross‐linking Induces Phosphorylation of p38 in CD4+CD45RO+ T Cells
Author(s) -
Tamma Seetha M.,
Balan S. P.,
Akshintala S. Deepika
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.663.9
Subject(s) - cd28 , phosphorylation , cd8 , cd3 , microbiology and biotechnology , p38 mitogen activated protein kinases , t cell , t cell receptor , chemistry , biology , mapk/erk pathway , antigen , immunology , immune system
CD27 antigen is a 120 KDa transmembrane protein and a member of nerve growth receptor family. Its expression is predominantly confined to lymphocytes and equally distributed between CD4 and CD8 lymphocytes. It has been reported that CD27 is required for long term maintenance of T cell immunity and was found to rescue CD28(−/−) T cells from death at the onset of division. We investigated the role of CD27 on resting and memory CD4+ T cells. We isolated human CD4+CD45RA+ and CD4+CDRO+ subsets using Dynal magnetic beads and cultures were set up by cross‐linking with antibodies as indicated: CD3 + CD28; CD27 alone; CD27 + CD3; CD27 + CD28; CD28 alone and control cells. MAPK ERK2 phosphorylation appears to be similar in both RA and RO populations when stimulated with CD27 plus CD28. We report that CD27 plus CD28XL resulted in enhanced and accelerated phosphorylation of p38 MAPK, activation peaked at 10 minutes and decreased by 2 hours in RO+ cells when compared with that in RA+ cells. CD27 on its own induced phosphorylation of ERK2 slightly higher than that in control, where as no phosphorylation of p38 was observed in both populations following cross‐linking with CD27 alone. This data suggests that RA and RO populations respond differently to signals transmitted through coreceptors CD27 plus CD28. Grant from C.W. Post Campus Research Committee of Long Island University, New York supported this work.