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Sensors of mitosis
Author(s) -
Morris May Catherine,
Scheper Johanna,
Pellerano Morgan
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.652.2
Subject(s) - mitosis , microbiology and biotechnology , cyclin dependent kinase 1 , cdc25 , cell growth , cell cycle , cyclin b1 , biology , phosphatase , cell , cyclin , chemistry , computational biology , phosphorylation , biochemistry
Detection of cell cycle regulators whose aberrant expression or activation influences cell cycle progression and cell proliferation is critical for the development of efficient diagnostic and therapeutic strategies. In particular Cdk1‐cyclin B1 and Cdc25 phosphatases are central players and partners that coordinate entry into mitosis and whose function is critical for cell proliferation. We have developed non‐genetically encoded fluorescent sensors which specifically recognize Cyclin B1 and Cdc25 phosphatases through molecular interfaces distinct from ATP‐binding pockets or active sites. In vitro characterization of these sensors reveals significant changes in their fluorescence upon specific detection of their target. The combination of these sensors with non‐covalent peptide‐based delivery systems enables efficient delivery into cultured mammalian cells. Characterization of the specificity and efficiency of these biosensors in a cellular environment provides promising perspectives for in vivo applications. Source of Research Support: CNRS & ANR Grant to MCM