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Effect of bioflavonoids on the proliferation of DU145 human prostate cancer cells
Author(s) -
JeanPierre Ninochka,
Faldu Gaurav,
Patel Suketu,
DePass Anthony,
Joseph Cecil K.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.648.26
Subject(s) - du145 , apigenin , myricetin , apoptosis , viability assay , dna fragmentation , cell growth , luteolin , rutin , pharmacology , chemistry , fragmentation (computing) , growth inhibition , cancer cell , cancer research , programmed cell death , microbiology and biotechnology , biology , biochemistry , flavonoid , medicine , cancer , antioxidant , kaempferol , lncap , ecology
Flavonoids are ubiquitously occurring and widely consumed secondary metabolites of plants and are therefore an integral part of the human diet. Apigenin, luteolin, Myricetin and Rutin are flavonoids that have various pharmacological activities including inhibitory effects on the growth and proliferation of tumor cells. We have investigated the effects of these flavonoids alone and in combination on the proliferation of androgen‐independent human prostate (DU145) cancer cells in culture. Our results indicate that apigenin, luteolin, myricetin and rutin inhibited cell proliferation and decreased the viability of DU145 cells after 96 hours of treatment. To determine whether the decreased viability and cell proliferation was due to cell death by apoptosis or necrosis, we performed western blot analysis using antibodies raised against the pro‐apoptotic protein Bad and the anti‐apoptotic proteins Bcl2 and Bcl xL , We observed a dose‐dependent and time dependent increase in the pro‐apoptotic Bad gene and a dose dependent and time dependent decrease in BcL 2 and Bcl xL when cells were treated with flavonoids for 72 hours. DNA fragmentation studies and expression of matrix metalloproteinase (MMP) – 2 using identical doses and time points confirmed the pro‐apoptotic effect of flavonoids on the proliferation of DU145 cells.