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Fatty acids regulate TNF‐α production by J774 cells
Author(s) -
Thais Martins de Lima,
Alice Cristina Rodrigues,
Camargo Rocha Michelle,
Curi Rui
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.643.10
Subject(s) - adipose tissue , chemistry , endocrinology , medicine , tumor necrosis factor alpha , arachidonic acid , population , stimulation , oleic acid , macrophage , inflammation , palmitic acid , fatty acid , biochemistry , biology , enzyme , in vitro , environmental health
Chronic inflammation has been reported in obesity‐related insulin resistance. Most of the adipose tissue‐derived cytokines, including TNF‐α, are originated from the infiltrated macrophage population. Macrophage accumulation has been associated to the increase in adiposity. However, molecular signals that regulate macrophage function in obese adipose tissue are not well known. The effect of various fatty acids (FA) on TNF‐α production by J774 cells (a model of murine macrophages) treated with 2.5μg/mL LPS was evaluated. Palmitic, stearic, oleic, linoleic, arachidonic, DHA and EPA acids were added at different concentrations 3 hours prior to LPS stimulation for 24 hours. Palmitic, stearic, DHA and EPA at 50μM, but not lower concentrations, increased LPS stimulatory effect on TNF‐α production. In the absence of LPS, all FA, with exception of oleic and linoleic acids, stimulated TNF‐α production, although TNF‐α concentrations in the medium were extremely lower. Real‐time PCR analysis showed that the stimulatory effect of FA on LPS induced TNF‐α expression occurred between 3 and 6 hours of treatment. NF‐κB activation was also increased after all FA treatments. These findings indicate that saturated and omega‐3 FA present similar effects on TNF‐α production by macrophages, via NF‐κB activation. Financial support: FAPESP, CNPq.