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CDK5: A Contributor to the Insulin Phenotype of Transdifferentiated β Cells
Author(s) -
Stevens Jessica Lee,
Aguanno Ann
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.638.3
Subject(s) - cyclin dependent kinase 5 , microbiology and biotechnology , kinase , biology , secretion , cell cycle , cellular differentiation , phenotype , insulin , cell , endocrinology , protein kinase a , cyclin dependent kinase 2 , biochemistry , gene
We investigate the role of CDK5 in mammalian development. Although CDK5 belongs to the cyclin dependent kinase family of proteins involved in regulating the cell cycle, CDK5 itself has been shown to influence differentiation of non‐dividing nervous tissues. Previously our lab has used PC12 cells to study CDK5's role in neuronal development. Experiments involving growth factor (NGF, FGF) induced differentiation of PC12 cells in the presence of a CDK5 inhibitor resulted in drastic morphological changes. Recent reports suggest CDK5 may also play a extra neuronal role. Therefore, we have explored the impact of CDK5 inhibition in pancreatic tissue using the acinar cell line AR42J as our model. We have investigated insulin production in the presence or absence of CDK5. To achieve this goal, the cells were differentiated into insulin‐producing cells by the administration of the chemical exendin‐4. CDK5 activity was inhibited by exposure to olomoucine, and the two flavonoids, kampherol and quercetin. Our results suggest that CDK5 plays a role in both the secretion and production of insulin. This research was supported by the Rose M. Badgley Charitable Trust, The Murray Foundation and the Division of Sciences, Marymount Manhattan College.