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Charge‐driven surface‐colloid interaction for detection biomolecules on microarrays.
Author(s) -
Sun Ye,
Golovlev Val
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.624.5
Subject(s) - biomolecule , surface charge , molecule , nanotechnology , dna microarray , chemistry , dna , particle (ecology) , surface modification , nanometre , rna , electrostatics , static electricity , nanoparticle , small molecule , biophysics , materials science , gene expression , gene , physics , biochemistry , organic chemistry , biology , quantum mechanics , ecology , composite material
The aim of this study is to demonstrate a new approach for high‐multiplexed analysis of RNA, DNA, and proteins without chemical modification of target molecules for detection. The approach utilizes nanometer‐sized particles, which carry electric charges and are attracted by electrostatic force to target molecules captured on a solid surface. The nano‐particles bind to the surface proportionally to the number of target molecules. Location and surface density of the bound particles can be measured by a low‐cost optical detection system. In our study the charge‐driven particle‐surface interaction was investigated using Gouy‐Chapman‐Stern‐Graham model. The model predicts experimental conditions at which nano‐particles bind selectively to large target molecules and do not bind to small capture molecules present on the same surface. Important implication of this result is the possibility to detect DNA, RNA, and proteins immobilized on a surface with no need for chemical modification or labeling of the target molecules. The detection approach has been demonstrated for gene expression analysis and for protein detection using microarrays. This study is supported by NIH grant 2R44GM074311.