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Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements
Author(s) -
Chang ChiaChing,
Lin PoYen,
Chang ChiaSeng,
Kan LouSing
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.624.3
Subject(s) - förster resonance energy transfer , macromolecule , chemical physics , intermolecular force , chemistry , molecule , dna , molecular dynamics , nanometre , atomic force microscopy , angstrom , fluorescence , nanotechnology , crystallography , materials science , computational chemistry , physics , biochemistry , organic chemistry , composite material , quantum mechanics
We have detected the dynamics of 17‐mer DNA‐triplex dissociation mechanism at the molecular level. Fluorescence resonance energy transfer (FRET) was used as an indicator of intermolecular interaction in nanometer range, whereas atomic force microscopy (AFM) was employed to address single molecule with sub‐angstrom precision. The maximum rupture force of DNA‐triplex was found at pH 4.65, consistent with nuclear magnetic resonance (NMR) observations. These results indicated that the FRET together with an AFM detection system could be used to reveal the DNA‐triplex interaction in nanometer scale unambiguously. We also find that the single molecule dynamic of bio‐macromolecule may represent the statistical reaction of macromolecule system.