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Popcorn‐Like Conformational Transition of Bio‐Nanoparticle Complex: Analysis and Application
Author(s) -
Lee TzuCheng,
Chou ChingChung,
Cheng PeiHsin,
Chang ChiaChing
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.622.2
Subject(s) - raman spectroscopy , fluorescence , chemistry , molecule , surface modification , irradiation , biophysics , cleavage (geology) , nanoparticle , materials science , nanotechnology , photochemistry , crystallography , optics , physics , organic chemistry , biology , fracture (geology) , composite material , nuclear physics
Nanometer sized diamond (ND), in addition to its noncytotoxicity, intrinsic fluorescence and easiness of surface functionalization, contains ‐C‐N=O functional group inside its structure. The ‐C‐N=O group can be photo‐cleaved by UV light and then releases NO molecule. Here, we use nanosecond pulse laser to trigger photo‐cleavage process based on the multi‐photon absorption. Comparing the Raman spectra before and after laser irradiation reveals the structure of ND changes conformation from sp 3 diamond structure to aromatic graphite‐like sp 2 bonded structure. Also, the AFM & SEM analyses show that 5‐nm NDs were expanded to ∼100 nm in diameter. Additionally, by conjugating ND with growth hormone(rEaGH), it can recognize cancer cell (A549) which frequently contains high copy number of growth factor receptors. Causing a “Popcorn‐like” conformational transition changes the cell's morphology and makes the cell apoptosis in consequence. Combining with those photophysical and biochemical properties mentioned above, we suggest that ND is potentially a good candidate in cancer therapies and other biomedical applications.