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Proteomic Approaches to Defining Avian Natural Killer Cell Surface Proteins
Author(s) -
Zhang Lei,
Katselis George S.,
Goto Ronald M.,
Goebel Thomas W.,
Yokoyama Wayne M.,
Lee Terry D.,
Miller Marcia M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.620.3
Subject(s) - biology , cell , innate immune system , membrane protein , natural killer cell , microbiology and biotechnology , receptor , computational biology , cytotoxic t cell , biochemistry , membrane , in vitro
Receptor/ligand interactions that dominate the control of natural killer (NK) cell activity likely differ in detail among vertebrate species as the result of natural selection. We are interested in innate immune responses in the avian species that serve as alternate hosts for pathogens causing human disease. We would like to link the challenge of pathogens to the genetics of NK cell activity. We are applying a largely unbiased high throughput proteomic approach to identify components of the surface of chicken NK cells. Our goal is to develop methods for preparing membrane fractions in abundance so that rare proteins, e.g. NK cell receptors, can be detected by mass spectrometry. We are comparing different fractionation methods for the capacity to provide membrane proteins from embryonic spleen cells that exhibit natural cytotoxic activity. We found it necessary to enhance the yield of membrane proteins since simpler methods are inadequate. We are now comparing methods incorporating fractionation by relative hydrophobicity and by shotgun glycopeptides capture. NK cell surface proteins identified by molecular biology serve as controls in evaluating purification. Supported in part by NSF MCB‐0524167