Premium
Novel Genotypes Relevant to Enhanced Resistance to γ‐irradiation in Escherichia coli
Author(s) -
Popelars Michael C.,
Hao Li,
Hamilton Lindsay L.,
Klugman Sarit A.,
Paider Jessica J.,
Wood Elizabeth A.,
Battista John R.,
Cox Michael M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.591.2
Subject(s) - deinococcus radiodurans , biology , population , genetics , escherichia coli , mutation , genotype , phenotype , ionizing radiation , mutation frequency , deinococcus , dna , gene , irradiation , medicine , physics , nuclear physics , environmental health
To better understand the extraordinary resistance to ionizing radiation displayed by bacterial species such as Deinococcus radiodurans , we have generated a series of E. coli populations that also have a greatly elevated capacity to survive exposure to ionizing radiation. One single colony isolate from each population was obtained and given a designation: CB1000, CB2000, and CB3000, taken from the first, second, and third evolved population, respectively. These isolates were independently subjected to comparative genome resequencing, revealing in each case the presence of 60–70 mutations1. To determine which of the CB1000 mutations was relevant to the radiation resistance phenotype, we subjected the population from which CB1000 was derived to ensemble sequencing. DNA from that population was PCR amplified at each region where a mutation was identified in CB1000. Those mutations that were common in the population could be identified in the resulting ensemble sequences. Those mutations incidental to the phenotype were generally not detectable in the ensemble sequences. Less than 5 mutations were found via the ensemble approach. These mutations and their effects on otherwise wild type E. coli strains will be reported on in this poster.