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Interaction between cardiac heme oxygenase and natriuretic peptide system
Author(s) -
Armstrong David William John,
Tse M. Yat,
Melo Luis G.,
Liu Xiaoli,
Pang Stephen C.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.589.1
Subject(s) - heme oxygenase , atrial natriuretic peptide , medicine , natriuretic peptide , messenger rna , endocrinology , spleen , genetically modified mouse , gene expression , transgene , heme , chemistry , biology , gene , enzyme , heart failure , biochemistry
Genetic mouse models have recently shown that cardiac heme oxygenase (HO) and natriuretic peptide system (NPS) are essential for proper tissue dynamics and gene expression in the heart. However, the interaction between these two systems has not yet been investigated. The objective of this study was to determine the effect of ANP disruption on gene expression of the HO system, and to determine the effect of HO‐1 ablation and cardiac‐specific HO‐1 over‐expression on the NPS. Three mouse models were utilized: ANP −/− , HO −/− and HO‐1 cardiac‐specific transgenic (HO‐1 TG), and the respective wild‐type controls. Organs were excised, weighed, and snap‐frozen in liquid N 2 . Total RNA was extracted and analyzed by real‐time PCR. As expected, ANP −/− mice exhibited significant left ventricular hypertrophy. Surprisingly, HO‐1 −/− mice exhibited splenomegaly (almost three fold increase in spleen mass). There was no significant difference in ventricular HO‐2 mRNA expression in ANP −/− mice, however ventricular HO‐1 mRNA expression was significantly lower in ANP −/− mice compared to wild‐type controls. These data support the notion that HO and NPS interact with each other. The significance and the mechanism of splenomegaly in the HO −/− mice remain to be elucidated. Supported by the Heart and Stroke Foundation of Ontario.