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3D‐Micro‐Ultrasound Documents Diltiazem Mediated Changes in Central Aortic Hemodynamics
Author(s) -
Bunzel Michelle M,
Campbell Barry,
Azer Karim,
Tong Christopher,
Ramcharan Sharmilee,
Saini Kavita,
Fancourt Craig,
Wang Shubing,
Walker Matthew
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.587.2
Subject(s) - diltiazem , medicine , blood pressure , hemodynamics , doppler imaging , cardiology , ultrasound , diastole , blood flow , abdominal aorta , aorta , anesthesia , radiology , calcium
3D‐micro‐ultrasound imaging has emerged as a valuable research tool to co‐register vascular physiology. This co‐registration allows serial interrogation of arterial wall integrity, blood flow and stiffness. We quantified hemodynamic changes associated with the acute administration of Diltiazem, to benchmark high throughput imaging preclinically. Six male SD rats implanted with DSI pressure probes in the femoral artery were imaged using the VisualSonics Vevo770 (25MHz probe) system. Baseline Doppler measurements in the abdominal aorta were acquired followed by administration of 3mpk Diltiazem i.v. Measurements were then acquired during the acute phase of drug administration and every 5’ over a 60’ time course. Administration resulted in an increase from baseline in cardiac period, diastolic time and Doppler peak‐to‐peak/valley‐to‐valley interval over 60’. Heart rate decreased relative to baseline as did the mean Doppler velocity and max dp/dt (positive/negative). Decreases in systolic BP, diastolic BP, MAP and pulse pressure were also seen. Our novel assessment of the anti‐hypertensive vascular and cardiac effects of Diltiazem is attributable to its primary indication and consistent with the literature. Evaluation of vascular structure and function in real, fiducially aligned, spatial and temporal resolution, unveils a powerful imaging tool for the in vivo characterization of compound efficacy.