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Function after repair of skeletal muscle defect with muscle‐derived extracellular matrix
Author(s) -
Merritt Edward K,
Walters Thomas J,
Baer David G,
Farrar Roger P
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.580.3
Subject(s) - extracellular matrix , skeletal muscle , isometric exercise , gastrocnemius muscle , muscle tissue , anatomy , myocyte , chemistry , medicine , biochemistry
The loss of a significant portion of skeletal muscle poses a unique challenge for the normally robust regenerative capacity of muscle tissue. A partial transection injury with tissue loss, which causes a large gap to develop in the muscle, will not fully heal as the fibers are unable to span the gap to reconnect the disconnected ends. In order to determine a possible functional repair technique of a skeletal muscle defect, a 1 x 1 cm portion of the lateral gastrocnemius of Lewis rats was removed. A skeletal muscle derived extracellular matrix (ECM) was implanted in place of the lost tissue. Without ECM repair, the maximal isometric tetanic force of the defected lateral gastrocnemius was 59.6% ± 4.7, 72.9% ± 2.3, and 78.0% ± 1.5 of the nonoperated contralateral limb 7, 14, and 28 days post‐injury. With ECM repair, the maximal force of the defected lateral gastrocnemius was 80.7% ± 8.4, 77.4% ± 4.2, and 84.0% ± 3.6 of the nonoperated limb. These data suggest that after 28 days of recovery from a defect injury, the lateral gastrocnemius will not recover full functionality. Addition of an extracellular matrix to fill the defect results in more force 7, 14, and 28 days post‐defect, but full recovery still does not occur. This work funded in part by the U.S. Army Medical Research and Material Command grant DAMD W81XWH‐06‐1‐0540 to RPF