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Stem/Progenitor Cell Mobilization for Tissue Regeneration
Author(s) -
Lee Sang Jin,
Van Dyke Mark,
Atala Anthony,
Yoo James
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.579.5
Subject(s) - progenitor cell , stem cell , regeneration (biology) , microbiology and biotechnology , endothelial stem cell , tissue engineering , regenerative medicine , in vivo , scaffold , biology , pathology , in vitro , medicine , biomedical engineering , biochemistry
In this study we explored the possibility of utilizing host stem or progenitor cells for tissue regeneration in vivo. We investigated whether these cells could be mobilized into target specific sites for regenerative medicine applications. Materials and Methods Polyglycolide polymers were implanted subcutaneously in mice to recruit host cells. The scaffolds were retrieved at 1, 2, 3 and 4 weeks after implantation for analyses. Cells contained within the retrieved scaffolds were cultured and differentiated into osteogenic, endothelial, myogenic, and adipogenic cell lineages using the respective cell differentiation conditions. Results and Discussion The retrieved implants showed a progressive increase in neovascularization and host cell infiltration with time. Tissue infiltrates within the scaffolds showed the evidence of collagen deposition after 2 weeks of implantation and continued to increase over time. The culture of infiltrated cells showed the expression of stem cell markers and these cells were able to differentiate into various cell lineages. Conclusions This study demonstrates that stem or progenitor cells can be mobilized and recruited into target specific sites. These findings suggest that host stem cells may be maneuvered to achieve tissue regeneration in vivo using a target specific scaffold system.