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Fascial Tissue Reconstruction Using Acellular Collagen Matrix
Author(s) -
Eberli Daniel,
Yoo James,
Atala Anthony
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.579.2
Subject(s) - viability assay , tissue engineering , matrix (chemical analysis) , biomaterial , in vivo , biocompatibility , medicine , extracellular matrix , decellularization , biomedical engineering , abdominal wall defect , anatomy , abdominal wall , surgery , urology , chemistry , apoptosis , biology , biochemistry , microbiology and biotechnology , organic chemistry , chromatography
Statement of Purpose: Acellular collagen matrices have been used experimentally and clinically for several applications, including urethra and bladder reconstruction. We investigated the feasibility of using a porcine collagen‐based matrix as an off‐the‐shelf biomaterial for fascial tissue repair in 3 different tissue systems. Methods: Biocompatibility of the acellular matrix derived from porcine bladders was assessed using cell viability, MTT and apoptotic index. The acellular collagen matrices were used as a sling material for the treatment of incontinence, abdominal hernias and penile tunica reconstruction in 24 rabbits. The animals were evaluated up to 3 months after implantation. Results / Discussion: The acellular matrix did not induce significant changes in cell viability, proliferation and apoptotic activity when compared with the controls. All animals survived without any untoward effects. The matrices remained intact at their respective implantation sites and demonstrated the maintenance of fascial tissue function. Histologically, there was only a minimal inflammatory response, which gradually decreased over time. Conclusions: These results show that the acellular collagen matrix derived from porcine bladders is biocompatible, durable and safe for use in vivo.