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Peripheral blood‐derived multipotent adult progenitor cells differentiate to neural phenotypes in vivo and in vitro
Author(s) -
Spitzer Nadja,
Butts Heather M.,
Samuel Sarah A.,
Price Elmer M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.578.4
Subject(s) - neun , progenitor cell , neural stem cell , microbiology and biotechnology , biology , stem cell , cell therapy , multipotent stem cell , transplantation , neurogenesis , pathology , immunology , medicine , immunohistochemistry
Cell therapy offers a promising approach in treating neurodegenerative disorders such as Parkinson's Disease (PD), and therapies utilizing adult‐derived stem cells are highly desirable. We have derived novel multipotent progenitor cell lines (PBD‐MAPCs) from the peripheral blood of adult green fluorescent protein (GFP) transgenic swine. Undifferentiated PBD‐MAPCs proliferate as suspended spheroids through >100 doublings. To demonstrate the neuronal potential of these cells, we injected them into the brains of neonatal (P0) rats. After 8 weeks, GFP+ cells with neuronal‐like morphology were localized primarily to the hippocampus and expressed β‐tubulin III, NeuN and other neuron‐specific proteins. Therefore, given appropriate differentiation signals provided by the environment in the neonatal brain, PBD‐MAPCs can develop neuronal phenotypes. In an effort to predifferentiate PBD‐MAPCs to a neural lineage in preparation for transplantation into animal models of PD, we cultured cells in media with defined factors. High proportions of these cells develop a neuronal morphology and express neuronal markers. Finally, PBD‐MAPC cells injected into the striatum of 6‐hydroxydopamine‐lesioned rats reduce apomorphine‐induced rotations; the injected cells therefore act to rectify PD‐like deficiencies. Supported by NSF EPSCoR EPS‐07‐02#2#EPS0554328.