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MOUSE CARDIAC MR IMAGING OF MAGNETICALLY LABELED HUMAN AMNIOTIC FLUID STEM CELLS FOR LONGITUDINAL CELL GRAFT MONITORING
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.577.3
Subject(s) - stem cell , magnetic resonance imaging , flow cytometry , pathology , fluorescence lifetime imaging microscopy , chemistry , cell , biomedical engineering , microbiology and biotechnology , medicine , fluorescence , biology , biochemistry , radiology , physics , quantum mechanics
We hypothesize that micrometer‐sized iron particles (MPIOs) can be used for tracing stem cells for therapeutic application We have tested high resolution Magnetic Resonance Imaging Magnetic Resonance Imaging (MRI) to track and monitor amniotic fluid derived stem (AFS) cells injected into mouse hearts. MPIO were introduced into human AFS cells using nucleofection and were detected both by fluorescence microscopy and MRI. Flow cytometry analysis was done to quantify the number of labeled cells over several cell divisions. MPIO labeled AFS cells were injected into the heart of mice and MRI was performed at varying times after cell injection. After MRI, histology was used to confirm the location of stem cells in the heart. Flow cytometry analysis revealed that MPIO labeled AFS cells maintain labeled for up to 14 divisions. MRI of agarose gels injected with MPIO labeled AFS cells demonstrated significant hypointense regions. Labeled AFS cells injected into mouse hearts demonstrated successful hypointense regions by MRI at 24 hours, 1, 2 weeks, 3 and 4 weeks. Results of AFS cell integration in the heart were further confirmed by histological analysis using fluorescence microscopy and Prussian blue staining. This study represents the first report of in vivo MRI identification and tracking of MPIO‐labeled stem cells in the mouse heart using MRI.