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In vitro migration of mesenchymal stem cells (MSC) promotes differentiation to chondrocytes
Author(s) -
Khoury Joseph,
Geuss Laura R.,
Shortkroff Sonya,
Berlowitz Tarrant Laurence J.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.522.5
Subject(s) - mesenchymal stem cell , chondrogenesis , chemistry , glycosaminoglycan , in vitro , microbiology and biotechnology , cellular differentiation , chondrocyte , andrology , biology , biochemistry , medicine , gene
The capacity of MSC to differentiate into numerous cell types, including chondrocytes, has been well studied. However, the effects on differentiation of MSC during migration have yet to be elucidated. To assess migration, MSC or articular chondrocytes (hAC) were seeded in the upper portion of 8μm Boyden chambers with 500μl medium containing 1% BSA, chondrogenic medium with TGFβ (CCM), or basal medium with 10% FBS in the lower portion in the presence or absence of collagen scaffolds (HC) (n=6). Chambers were incubated for 16h prior to counting the migrated cells. To assess differentiation after migration, cells were plated in the upper chamber and allowed to attach for 48h. Then, HC discs were placed in the lower chamber with CCM or basal medium and chambers were cultured for 2 weeks; cell seeded HC discs served as controls (n=9). HC were assayed for sulfated glycosaminoglycan (S‐GAG) accumulation; processed for histology; total RNA was extracted and amplified by RT‐PCR. MSC and hAC preferentially migrated towards wells that contained HC (p<0.01). MSC that migrated produced 70% more S‐GAG than control and 2.43 fold more than hAC (p<0.05). By RT‐PCR, MSC that migrated into HC increased COL2A1 and AGC1 expression while hAC showed signs of dedifferentiation with increased COL1A1. This study reveals that migration of MSCs into a collagen scaffold can enhance differentiation into chondrocytes and ECM production.